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Paraffin wax section is a practical granular paraffin wax suitable for impregnation, embedding and sectioning of tissue samples. High-quality paraffin wax and standard polymers are combined by advanced technology. The slicing is smooth, and the thickness can be as thin as 2 μm. The melting point ranges from 52°C to 62°C, which can be freely selected according to your needs.
The paraffin wax used for embedding should have a melting point between 50°C and 60°C, which should be selected based on the hardness of the material, the thickness of the slice, and the temperature conditions at the time. The most commonly used melting points for animal materials are 52-56°C, while plant materials use 54-58°C; thinner slices use 58-60°C and thicker slices use 52-54°C. The paraffin wax with a melting point of 52-54°C can be used to smoothly slice at a room temperature of 10-19°C. In winter, paraffin wax with a melting point of 46-48°C can be used, while in summer, a melting point of 56-58°C can be selected.
Embedding is a process of wrapping tissue blocks impregnated with wax in paraffin wax. The specific procedure is: prepare the mold first, place the tissue flat on the bottom of the mold, face down, cover the embedding box on the mold, and pour the melted wax into the mold until the wax overflows from the mold. Then gently lift the mold, place it flat in the freezing table, and take it out after it solidifies quickly for about 10min.
The temperature of the wax used for embedding should be slightly higher than the temperature of penetration wax to ensure that the tissue blocks are completely integrated with the surrounding paraffin wax. Rapid cooling of paraffin wax is also essential; otherwise, crystallization will occur in the embedded block when sliced later, causing fragmentation.
Precautions
Store below 35°C and avoid direct sunlight.
High-temperature storage may cause paraffin wax to clump, but it does not affect its use.
Paraffin wax is a hydrocarbon compound produced by the splitting of mineral oil. Its properties vary greatly, with melting points ranging from 45°C to 75°C. Paraffin wax with a lower melting point is milky white and fragile, while that with a higher melting point is pale yellow, flexible to hard, and not easily broken.
The preferred form of paraffin wax use is emulsified wax, which has the characteristics of acid and alkali resistance, strong water solubility, stable emulsion, long shelf life, high solid content, and good dispersibility. It can be used in grinding, papermaking, leather, wood, agriculture, explosives, medicine, ceramics, cosmetics, and automobile protection industries and fields.
The use of paraffin wax section in medicine is an essential embedding agent for animal and plant tissue sections' technical application, which is the basic material for biology, medicine, pathology, and immunohistochemistry.
Currently, China has a wide variety of paraffin wax section types. High-melting-point paraffin is harder and more challenging to slice, and the flexibility is not suitable for thinner slices. Low-melting-point paraffin wax has lower hardness, and the support for slicing is insufficient, making it difficult to cut thinner slices.
Therefore, to improve the quality of medical paraffin wax in China and enhance its competitiveness in the market, the development and research of medical paraffin have become increasingly important. Due to the wide range of melting points of paraffin wax, there are many choices. Therefore, it is necessary to choose paraffin wax suitable for local and unit use to produce high-quality pathological tissue sections and various tissue sections. If the melting point of the selected paraffin wax is too high, the paraffin wax's hardness will be too hard, making it difficult to slice and not suitable for flexibility. If the paraffin wax's melting point is lower, the hardness will also decrease, making it difficult to support the slice, and difficult to cut thin slices.
We highly recommend King Honor's Paraffin Wax for all your wax-related needs. Our paraffin wax products are of exceptional quality and provide excellent results for various applications, from candle making to skincare and industrial uses. Welcome to contact us!
